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Pakistan Journal of Zoology, 48(3), 853–859

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The GC temperature program started with a 1 min hold at 40 °C followed by a 6 °C/min ramp up to 210 °C, a 20 °C/min ramp up to 330 °C and a bake-out at 330 °C for 5 min using Helium as carrier gas with constant linear velocity